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本帖最后由 little_joe 于 2016-06-15 16:21 编辑
文件A:
WP_000636925.1-1
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
WP_000567766.1-1
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
77
79
79
79
80
80
80
80
80
80
WP_000636925.1-1
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
63
62
WP_001341795.1-1
24
24
24
24
24
文件B:
BLASTP 2.2.29+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
Query= WP_001386572.1-1
Length=21
***** No hits found *****
Lambda K H a alpha
0.314 0.134 0.424 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1206911076
Query= WP_000636925.1-1
Length=105
Score E
Sequences producing significant alignments: (Bits) Value
lcl|CU928161.2_prot_CAR03545.1_2173 [gene=ECS88_2260] [protein=c... 104 2e-29
>lcl|CU928161.2_prot_CAR03545.1_2173 [gene=ECS88_2260] [protein=conserved hypothetical protein] [protein_id=CAR03545.1]
[location=2240570..2240740]
Length=56
Score = 104 bits (260), Expect = 2e-29, Method: Compositional matrix adjust.
Identities = 49/56 (88%), Positives = 52/56 (93%), Gaps = 0/56 (0%)
Query 8 LLFTIMRSLYLKIRFPSDLRSINALNYLQVPSIDWSDSGYVQHFINVIEKMLTRKK 63
+LFTIMRSLYLK RFPSDL+SINALNYLQVPSIDWSDSGY+QHFINVIEKM T K
Sbjct 1 MLFTIMRSLYLKSRFPSDLKSINALNYLQVPSIDWSDSGYIQHFINVIEKMPTTNK 56
Lambda K H a alpha
0.325 0.135 0.407 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1406358592
Query= WP_000636925.1-1
Length=105
Score E
Sequences producing significant alignments: (Bits) Value
lcl|CU928161.2_prot_CAR03545.1_2173 [gene=ECS88_2260] [protein=c... 104 2e-29
>lcl|CU928161.2_prot_CAR03545.1_2173 [gene=ECS88_2260] [protein=conserved hypothetical protein] [protein_id=CAR03545.1]
[location=2240570..2240740]
Length=56
Score = 104 bits (260), Expect = 2e-29, Method: Compositional matrix adjust.
Identities = 49/56 (88%), Positives = 52/56 (93%), Gaps = 0/56 (0%)
Query 8 LLFTIMRSLYLKIRFPSDLRSINALNYLQVPSIDWSDSGYVQHFINVIEKMLTRKK 63
+LFTIMRSLYLK RFPSDL+SINALNYLQVPSIDWSDSGY+QHFINVIEKM T K
Sbjct 1 MLFTIMRSLYLKSRFPSDLKSINALNYLQVPSIDWSDSGYIQHFINVIEKMPTTNK 56
Lambda K H a alpha
0.325 0.135 0.407 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 1406358592
通过文件A中的WP_000636925.1-1匹配到文件B:Query= WP_000636925.1-1
读取文件A下面的数值,该数值将作为序号查找文件BQuery行即红色行对应的内容
Query行刚开头的8即为第一个字母L的序号,则62,63对应Query行中的KK
输出为Sbjct行即蓝色行中Query找出的KK对应的NK
并且分别计算输出的NK的个数
例如:
N:2
K:2 |
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